[Sunflower]

J Bacteriol. 2005 March; 187(5): 1799–1814.
PMCID: PMC1063990

Screening for Quorum-Sensing Inhibitors (QSI) by Use of a Novel Genetic System, the QSI Selector

Thomas Bovbjerg Rasmussen,1 Thomas Bjarnsholt,1 Mette Elena Skindersoe,1 Morten Hentzer,2 Peter Kristoffersen,1 Manuela Köte,3 John Nielsen,4 Leo Eberl,3,5 and Michael Givskov1,*

Effect on biofilm tolerance to tobramycin.

It has previously been shown that P. aeruginosa biofilm cells are highly tolerant to antibiotic treatment (3). Davies et al. (9) demonstrated that a QS mutant of PAO1 is more susceptible to sodium dodecyl sulfate (SDS) treatment than the wild-type counterpart. Further, Hentzer et al. (25) showed that biofilms treated with a QSI became susceptible to both SDS and tobramycin.

We tested if garlic extract exhibited a similar effect. Two sets of PAO1 biofilms were allowed to form for 3 days in flow chambers in which the medium contained either no garlic or 1% garlic. Following this, each biofilm was challenged with 340 g of tobramycin/ml for 24 h. The effect of the antibiotic treatment was assessed by live/dead staining.

In Fig. 5D, it can be seen that most of the cells in the biofilm treated with both garlic and tobramycin died (Fig. 5D).

In striking contrast, only the cells in the top layer of the biofilm treated with tobramycin alone were killed (Fig. 5B).

Further, Fig. 5C shows that treatment with garlic extract alone had no effect on biofilm viability.

Comparing the untreated biofilm (Fig. 5A) with a garlictreated one (Fig. 5C), a difference in architecture is observed.
The untreated biofilm exhibited the classical mushrooms of a P. aeruginosa biofilm grown in glucose-containing medium. A
flatter, undifferentiated biofilm was formed when the medium contained 1% garlic extract. This effect would be expected by
QSI treatment, since a QS-defective lasI mutant of PAO1 has been found to form flat, undifferentiated biofilms (9).

Fig.5:
http://www.ncbi.nlm.nih.gov/pmc/articles...figure/f5/

Grün = lebende Bakterien
Rot= tote Bakterien

A = Biofilm nicht behandelt
B = Biofilmnur mit Tobramycine behandelt
C= Biofilm nur mit Knoblauch behandelt
D= Biofilm mit Tobramycine + Knoblauch behandelt

Ajoene, a Sulfur-Rich Molecule from Garlic, Inhibits Genes Controlled by Quorum Sensing

Tim Holm Jakobsena, Maria van Gennipa, Richard Kerry Phippsb,
Meenakshi Sundaram Shanmughamc, Louise Dahl Christensena,
Morten Alhedea, Mette Eline Skindersoed, Thomas Bovbjerg Rasmussene, Karlheinz Friedrichf, Friedrich Uthef, Peter Østrup Jenseng, Claus Moserg, Kristian Fog Nielsenb, Leo Eberlh, Thomas Ostenfeld Larsenb, David Tannerc, Niels Høibya, Thoma Bjarnsholta, and Michael Givskova,

http://aac.asm.org/content/56/5/2314.abstract


Oxford Journals
Medicine
Journal of Antimicrobial Chemotherapy
Volume 67, Issue 8
Pp. 1915-1926.

Antimicrobial effect of diallyl sulphide on Campylobacter jejuni biofilms

Xiaonan Lu1,2,3, Derrick R. Samuelson1, Barbara A. Rasco2 and
Michael E. Konkel1

http://jac.oxfordjournals.org/content/67...5.abstract

Garlic ointment inhibits biofilm formation by bacterial pathogens from burn wounds

Pushpalatha Nidadavolu1, Wail Amor2, Phat L. Tran3, Janet Dertien4, Jane A. Colmer-Hamood1 and Abdul N. Hamood1

http://jmm.sgmjournals.org/content/61/Pt_5/662.abstract

Is allicin able to reduce Campylobacter jejuni colonization in broilers when added to drinking water?

J. Robyn*, G. Rasschaert*,1, D. Hermans†, F. Pasmans† and
M. Heyndrickx

http://ps.fass.org/content/92/5/1408.abstract

Dr.Klinghardt, Dr.Zhang und Dr.B (Deutschland) verwenden Knoblauch-Extrakte in der Behandlung der chronischen Borreliose.Borrelia burgdorferi kann nachgewiesenermaßen in Biofilmen leben.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480481/

http://www.molecularalzheimer.org/files/...-Only_.pdf

http://www.youtube.com/watch?v=a4uNDWdChM8